Figure 1

Body weight, fat mass and energy balance in CB₁^+/+ and CB₁^-/- mice fed chow, a HF or a HF/FO diet during 6 weeks. A, Body weight development during the dietary intervention. B, Total fat mass at the end of the dietary intervention. C, Caloric intake, determined by manual food weighing, and averaged over the third to fifth week of dietary intervention. D, Fecal energy loss and E, Energy expenditure, derived from indirect calorimetry. Open symbols/bars, CB ₁ ^+/+ mice; closed symbols/bars, CB ₁ ^-/- mice. Values are given as means ± SEM for n = 5-13; # p < 0.05 compared to chow group of the same genotype; $ p < 0.05 compared to HF group of the same genotype, * p < 0.05 CB ₁ ^-/- vs. CB ₁ ^+/+ (Student t-test). General linear model analysis revealed overall effects for the following parameters (p < 0.05): Genotype: body weight at every timepoint, total fat mass, 24 h-energy expenditure. Chow versus HF: total fat mass, caloric intake, 24 h-energy expenditure. Chow versus HF/FO: total fat mass, caloric intake, fecal energy loss. HF versus HF/FO: caloric intake, fecal energy loss.