Figure 3

Fractional fatty acid synthesis rates and lipolytic activities in CB₁^+/+ and CB₁^-/- mice fed chow, a HF or a HF/FO diet during 6 weeks. A, Acetyl-CoA precursor pool enrichment. B, De novo palmitate synthesis. C De novo stearate synthesis. D, De novo oleate synthesis. E, Stearate synthesis from pre-existing palmitate. F, Oleate synthesis from pre-existing palmitate. G, Post-heparin plasma LPL activity and H, Adipose tissue-derived LPL activity normalized for protein content. Open bars, CB ₁ ^+/+ mice; closed bars, CB ₁ ^-/- mice. Values are given as means ± SEM for n = 4-7; # p < 0.05 compared to chow group of the same genotype; $ p < 0.05 compared to HF group of the same genotype (Student t-test). General linear model analysis revealed overall effects for the following parameters (p < 0.05): Chow versus HF: acetyl-CoA pool enrichment, de novo palmitate/stearate/oleate synthesis. Chow versus HF/FO: de novo palmitate/stearate synthesis. HF versus HF/FO: acetyl-CoA pool enrichment, de novo stearate/oleate synthesis.