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Table 1 Body composition and plasma metabolite concentrations in CB 1 +/+ and CB 1 -/- mice fed chow, a HF or a HF/FO diet during 6 weeks

From: Resistance to diet-induced adiposity in cannabinoid receptor-1 deficient mice is not due to impaired adipocyte function

 

chow

HF

HF/FO

 

CB 1 +/+

CB 1 -/-

CB 1 +/+

CB 1 -/-

CB 1 +/+

CB 1 -/-

Subcutaneous fat (g)

0.9 ± 0.1

0.7 ± 0.0

2.1 ± 0.3#

1.1 ± 0.1#*

2.5 ± 0.4#

1.4 ± 0.1#*

Epididymal fat (mg)

319 ± 40

227 ± 19

716 ± 113#

361 ± 34#*

644 ± 85#

406 ± 64#*

Retroperitoneal fat (mg)

116 ± 17

66 ± 6*

217 ± 33#

108 ± 7#*

300 ± 54#

145 ± 19#*

Brown fat (mg)

45 ± 6

41 ± 5

47 ± 6

36 ± 3

52 ± 5

36 ± 5

Mesenteric fat (mg)

194 ± 18

148 ± 12

303 ± 36#

181 ± 11*

336 ± 47#

190 ± 14*

Muscular fat (g)

0.6 ± 0.0

0.5 ± 0.0*

1.0 ± 0.1#

0.7 ± 0.0#*

1.1 ± 0.1#

0.8 ± 0.0#*

Organ fat (mg)

113 ± 15

103 ± 14

176 ± 28

161 ± 15#

238 ± 30#

133 ± 14*

Lean mass (g)

5.2 ± 0.6

4.6 ± 0.1*

5.0 ± 0.1

4.7 ± 0.1

5.0 ± 0.1

4.7 ± 0.1

Plasma metabolites and adipokines

     

Triglycerides (μM)

333 ± 31

245 ± 31

277 ± 29

275 ± 33

240 ± 63

183 ± 19

Cholesterol (mM)

1.3 ± 0.2

0.9 ± 0.1

1.9 ± 0.1#

1.8 ± 0.1#

1.4 ± 0.1$

1.3 ± 0.1#$

Non-esterified fatty acids (μM)

607 ± 32

391 ± 34*

586 ± 40

588 ± 51#

464 ± 38#

372 ± 26$

Blood glucose (mM)

9.3 ± 0.5

7.7 ± 0.8

9.1 ± 0.3

7.4 ± 0.4*

9.0 ± 0.4

8.1 ± 0.3

Insulin (pg/mL)

334 ± 53

356 ± 86

325 ± 39

270 ± 64

371 ± 82

300 ± 84

Leptin (ng/mL)

0.7 ± 0.1

0.6 ± 0.1$

2.1 ± 0.5#*

1.0 ± 0.2#*

2.2 ± 0.5#

1.7 ± 0.3#

Adiponectin (μg/mL)

14.3 ± 1.3

18.5 ± 2.4

15.4 ± 1.5

17.0 ± 2.9

18.1 ± 2.2

19.1 ± 2.2

Resistin (ng/mL)

2.3 ± 0.2

2.5 ± 0.4

3.0 ± 0.5

2.9 ± 0.3

2.1 ± 0.2

2.6 ± 0.4

  1. Values are given as means ± SEM for n = 6-8; # p < 0.05 compared to chow group of the same genotype; $ p < 0.05 compared to HF group of the same genotype, * p < 0.05 CB 1 -/- vs. CB 1 +/+ (Student t-test).
  2. General linear model analysis revealed overall effects for the following parameters (p < 0.05):
  3. Genotype: subcutaneous/epidydimal/retroperitoneal/brown/mesenteric/muscular/organ fat mass, lean mass, non-esterified fatty acid levels, glucose levels.
  4. Chow versus HF: subcutaneous/epididymal/retroperitoneal/mesenteric/muscular/organ fat mass, leptin levels.
  5. Chow versus HF/FO: subcutaneous/epididymal/retroperitoneal/mesenteric/muscular/organ fat mass, cholesterol levels, leptin levels.
  6. HF versus HF/FO: non-esterified fatty acid levels.
  7. Interaction between genotype and diet: non-esterified fatty acid levels.