Figure 2

Group mean mitochondrial content ±SD. A- Flow cytometric group mean log fluorescence of rhabdomyosarcoma cells measured in triplicate using 10,000 cells per sample, treated with OEP or CHD at 90 μg/ml (High) or 45 μg/ml (Low). B- Immunofluorescent quantification of cells treated as described above for 24 hours using 7 cells per treatment (n = 7). C- Confocal microscopy images of cells treated as described above for 24 hours. NOTES: Dapi nuclear stain (blue), mitochondrial stain (green), and red line represents 50 μm. * indicates p < 0.05, ** indicates p < 0.01 and *** indicates p < 0.001.