Figure 5

Acute activation of AMPK in gastrocnemius muscle. A. AMPKα2 activity in resting limb did not increase with iron deficiency. AMPKα2 increased with stimulation in both C and ID groups (* indicates statistical significance, p<0.05) (n=9-10). B. AMPKα1 activity in resting limb did not increase with iron deficiency. AMPKα1 activity in stimulated limb did increase with iron deficiency (ǂ indicates statistical significance, p<0.01). Also, activity of α1 did not increase with muscle stimulation in the C rats, whereas it did increase with stimulation in the ID rats (* indicates statistical significance, p=0.05) (n=8-10). C. Total AMPK phosphorylation did not significantly increase with iron deficiency. It did however increase with stimulation in both the C (* indicates statistical significance, p=0.05) and ID groups (ǂ indicates statistical significance, p<0.01) (n=3-4). D. Phosphorylation of ACC, a direct target of AMPK, did not significantly increase with iron deficiency, but did increase with stimulation in both C and ID groups (ǂ indicates statistical significance, p<0.01) (n=6-8).