Figure 6

AMPK alpha subunit expression in gastrocnemius muscle with iron deficiency anemia. A. AMPKα2 protein content decreased with iron deficiency (* indicates statistical significance, p<0.05) (n=5-6). B. AMPKα1 protein content was not different between the two groups (n=8). C. Total AMPK pan α protein content did not change with iron deficiency (n=4). The decrease in total AMPKα is not surprising given that α2 is the more abundant isoform found in skeletal muscle. The decrease in α2, with no concurrent decrease in α1 indicates a slight shift in α subunit composition due to iron deficiency.