Adipose tissue mass, SVF cell and Sca-1+/CD45-/CD34+cell numbers are increased in ob/ob mice. (A). Adipose tissue mass of C57BL/6J (lean) and ob/ob (leptin-deficient) mice. (B). Stromal vascular fraction (SVF) cells were isolated from epididymal white adipose tissue from lean and ob/ob mice and SVF cell numbers were quantified using FACS analysis. (C). CD34+ cells isolated using magnetic column were stained for Sca-1 and CD45 and quantified using FACS analysis. For (A)-(C), the mean ± SE for eight mice per group are plotted, *p<0.05 compared to lean mice.