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Figure 4 | Nutrition & Metabolism

Figure 4

From: Leptin differentially regulate STAT3 activation in ob/ob mouse adipose mesenchymal stem cells

Figure 4

Leptin induces MCP-1 secretion and mRNA expression in adipose stem cells in dose- and time-dependent manner. (A). The ob/ob mice Sca-1+/CD45-/CD34+ cells seeded as 1 × 106/ml/well in a 24-well culture plate and incubated with leptin (1000 ng/ml) for 24, 48, and 72 h. The supernatants were collected and MCP-1 protein levels were determined using ELISA. Data are expressed as mean ± SD, n=3. **p<0.01 compared to the value for untreated control cultures. ##p<0.01 compared to the value for treated with leptin 24 hours cultures. (B). The ob/ob mice Sca-1+/CD45-/CD34+ cells were seeded as 5×106/5ml/well in a 6-well culture plate and incubated for 6 h with various doses of leptin as indicated. (C). The cells were seeded in a same density as in (B) with or without leptin (1000 ng/ml) for 3, 6, 12, and 24 h. For (B) and (C), total RNA were extracted and relative MCP-1 mRNA expression was determined by real time PCR and normalized against 18S-rRNA. Data are expressed as mean ± SD, n=3. For (B) and (C), *p<0.05, **p<0.01 compared to the value for untreated control cultures and ##p<0.01 compared to the value for cultures treated with leptin for 3 h cultures.

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