Figure 6

EGCG treatment of isolated islets of Langerhans ex vivo fails to prevent palmitate-induced phosphorylation of JNK and formation of cleaved caspase 3. Pancreatic islets were isolated from lean C57Bl/6J mice and exposed ex vivo for 24 hours in the absence or presence of 0.5 mM palmitate with or without 5, 10 or 20 μM EGCG. Western blot analyses for phosphorylation of JNK1 (A), JNK2 (B) and formation of the apoptosis marker cleaved caspase 3 (C). Bars represent mean ± SEM for 5 different islet preparations in each group. * denotes P < 0.05 for a palmitate effect, and # denotes P < 0.05 for an EGCG effect using one-way ANOVA in conjunction with Dunnett's multiple comparison test. Representative blots for phosphorylated JNK1 (p-JNK1), phosphorylated JNK2 (p-JNK2) and cleaved caspase 3 (CC3) are shown in D.