Role of nitric oxide in the impaired myogenic response measured in arteries from Chowv ( n = 6), HSD ( n = 6) and HFD ( n = 6) rats. Experimental vessels were pre-treated with the general NOS inhibitor LNNA (100 μM). Data from untreated Chow, HSD and HFD arteries repeated from Figure 2 for comparison. Plateaus in responses at which point no further increases were seen are indicated by arrows and were determined from the two-way RM-ANOVA within group analyses. #p < 0.05 from untreated HSD or HFD control values at the same pressure step, respectively. Data expressed as mean ± SEM.