Effect of HFdiet and Kupffer cells reduction on lysosomal lipase activity measured as FFA release from artificial substrate (3H-triolein). A: light lysosomes; B: dense lysosomes. The lipase activity was measured as the release of fatty acids at pH = 4.5 from3H triolein. All data are presented together with median. Open symbols = starved animals (S); closed symbols = fed animals (F). ○ ● SD; □ ■ SD + GdCl3; ♦ ◊ HF; ▲ Δ HF + GdCl3. # p < 0.05 SD + GdCl3vs SD; *p < 0.05, *** p < 0.001 HF + GdCl3 vs HF; xp < 0.05 HF vs SD.