The effect of HF diet and Kupffer cells reduction on PKCε activation in the liver. Representative Western blot is shown in the upper part of the figure. The PKCε activation was assessed according to its translocation from cytosolic to membrane fraction and expressed as the ratio of relative densities of the bands in the membrane fraction and the corresponding ones in cytosolic fraction. All data are presented together with median. gray ○ SD; gray □ SD + GdCl3; gray ◊ HF; gray Δ HF + GdCl3. xxp < 0.01 SD vs HF; *** p < 0.001 HF + GdCl3 vs HF.