Effect of HF diet and Kupffer cells reduction on macrophage classical and alternative activation markers. A: HF vs SD group; B: SD + GdCl3 vs SD group; C: HF + GdCl3 vs SD group. GdCl3 was administered in two doses 72 and 24 hrs prior the experiment (10 mg/kg, i.p.). The expression of individual macrophage markers mRNA was determined by RT-PCR. Data are expressed as fold change related to untreated SD group with expression in standard diet (SD) arbitrary set at 1 (dotted line). gray ○ SD; gray □ SD + GdCl3; gray ◊ HF; gray Δ HF + GdCl3. xxp < 0.01 SD vs HF; #p < 0.05, ### p < 0.001 SD + GdCl3 vs SD; *p < 0.05, **p < 0.01, ***p < 0.001 HF + GdCl3 vs HF.