Comparison of effects of fasting and non-fasting on body weights, circulating blood VLDL triacylglycerol levels, and portal plasma glucose levels in rats fed the SD, SD + I, HF, or HF + I diet. Rats (7 weeks of age) were fed each diet for 3 weeks. The rats were sacrificed about 4 h after removal of the diet (non-fasting) or after starvation for 16 h. Values are the mean ± S.E. of 4 determinations. *p < 0.05, ***p < 0.001 versus SD diet-fed control rats; # p < 0.05, ###p < 0.001 versus HF diet-fed control rats. Open bar, non-fasting animals; closed bar, fasting animals.