Western blot analysis of NF-κBp65, IκB, Nrf2 and 4-HNE protein adducts in kidney homogenates of controls and chromium-treated rats. Densitometric analyses of these bands are represented as percent of control. Values are means ± standard error of the mean. Blots were repeated at least 3 times (n = 3) and a representative blot is shown. Actin was included to ensure equal protein loading. Data points with different superscripts are significantly different at the level of P < 0.05 by Fisher's comparison test. Control: no treatment; CrPic: Fed with standard diet and received CrPic; C + CrHis: fed with standard diet (8% fat) and received CrHis; HFD + STZ: received a HFD (40% fat) for 2 weeks and then were injected with streptozotocin (STZ) on day 14 (STZ,40 mg/kg i.p); HFD + STZ + CrPic: Treated with HFD/STZ but supplemented with CrPic. HFD + STZ + CrHis: Treated with HFD/STZ but supplemented CrHis. The mean bars with different letters differ (P < 0.05).