Figure 4

DHA reverses PA-induced lipid accumulation through repressing NLRC4 expression. (A) Generation of a HepG2 cell model with specific NLRC4 knockdown (NLRC4^+/−). NLRC4 and β-actin protein levels were evaluated by immunoblotting. (B) Representative Oil Red O staining of wild-type (WT) and NLRC4^+/− HepG2 cells were treated with different dose of PA with or without DHA preincubation wild-type (WT) and NLRC4^+/− cells are shown. Cells were examined by light microscopy at a magnification of 400×; (C) Intracellular TG content was measured by an ELISA assay. TG concentration was normalized by protein content. Data are shown as the mean ± S.E.; n = 4. *P < 0.05, **P < 0.01 compared with untreated cells; #P < 0.01 versus 0.4 mM PA-treated cells.