Random-fed insulin tolerance test. Glucose levels following insulin challenge in (A) male mice and (B) female mice at 0,15,30,45 and 60 minutes after challenge. Dissimilar means are denoted by **,*** indicating p-values of < .01 and < .001. Area Under the Curve (AUC GLUCOSE) as a measure of insulin resistance in (C) males and (D) females. Statistically significant differences are shown using different letters abc.