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Table 2 Respiratory parametersa of isolated rat brain mitochondria with pyruvate, D-lactate, or L-lactate or as combination.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb State 4 State 3 RCR ADP/O
No substrate 3.56 ± 0.31 5.21 ± 0.14 1.49 ± 0.11  
Cocktailc 4.51 ± 0.49 29.43 ± 2.92* 6.61 ± 0.73* 2.92 ± 0.01
Pyruvate 3.59 ± 0.21 30.98 ± 2.42* 8.46 ± 0.55* 2.12 ± 0.15
D-lactate 3.18 ± 0.16 7.64 ± 0.56 2.39 ± 0.10 -
L-lactate 3.18 ± 0.16 16.13 ± 0.49* 4.66 ± 0.17* 1.88 ± 0.10
Pyruvate + LLA 3.16 ± 0.45 22.09 ± 4.42* 7.16 ± 1.57* -
Pyruvate + DLA 4.07 ± 0.51 16.41 ± 2.85 3.94 ± 0.40 -
LLA + DLA 3.40 ± 0.34 5.41 ± 0.48 1.60 ± 0.08 -
  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate), DLA or LLA (5 mM + 2.5 mM malate), and LLA+DLA (2.5 mM LLA and 2.5 mM DLA+ 2.5 mM malate).
  3. cCocktail contains 62.5 μeach of malate, glutamate, alpha-ketoglutarate and pyruvate.
  4. State 4: The respiration state without ADP.
  5. State 3: The respiration state with the addition of ADP.
  6. RCR: Respiratory Control Ratio (state 3 rate:state 4 rate).
  7. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  8. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test, α < 0.05.