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Table 2 Respiratory parametersa of isolated rat brain mitochondria with pyruvate, D-lactate, or L-lactate or as combination.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb

State 4

State 3

RCR

ADP/O

No substrate

3.56 ± 0.31

5.21 ± 0.14

1.49 ± 0.11

 

Cocktailc

4.51 ± 0.49

29.43 ± 2.92*

6.61 ± 0.73*

2.92 ± 0.01

Pyruvate

3.59 ± 0.21

30.98 ± 2.42*

8.46 ± 0.55*

2.12 ± 0.15

D-lactate

3.18 ± 0.16

7.64 ± 0.56

2.39 ± 0.10

-

L-lactate

3.18 ± 0.16

16.13 ± 0.49*

4.66 ± 0.17*

1.88 ± 0.10

Pyruvate + LLA

3.16 ± 0.45

22.09 ± 4.42*

7.16 ± 1.57*

-

Pyruvate + DLA

4.07 ± 0.51

16.41 ± 2.85

3.94 ± 0.40

-

LLA + DLA

3.40 ± 0.34

5.41 ± 0.48

1.60 ± 0.08

-

  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate), DLA or LLA (5 mM + 2.5 mM malate), and LLA+DLA (2.5 mM LLA and 2.5 mM DLA+ 2.5 mM malate).
  3. cCocktail contains 62.5 μeach of malate, glutamate, alpha-ketoglutarate and pyruvate.
  4. State 4: The respiration state without ADP.
  5. State 3: The respiration state with the addition of ADP.
  6. RCR: Respiratory Control Ratio (state 3 rate:state 4 rate).
  7. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  8. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test, α < 0.05.
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Nutrition & Metabolism

ISSN: 1743-7075

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