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Table 3 Respiratory parametersa of isolated rat heart mitochondria with pyruvate, D-lactate, L-lactate alone or as combination as substrates.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb

State 4

State 3

RCR

ADP/O

No substrate

3.07 ± 0.15

7.72 ± 0.55

2.52 ± 0.08

 

Cocktailc

11.35 ± 1.52*

70.89 ± 5.89*

6.39 ± 0.59*

2.76 ± 0.02

Pyruvate

12.51 ± 1.19*

74.74 ± 6.22*

5.95 ± 0.22*

3.01 ± 0.02

D-lactate

4.62 ± 0.19

9.27 ± 1.31

2.01 ± 0.27

-

L-lactate

4.58 ± 0.58

15.17 ± 2.67

3.24 ± 0.21

2.11 ± 0.34

Pyruvate + LLA

4.30 ± 0.25

18.23 ± 1.52

4.23 ± 0.15*

-

Pyruvate + DLA

5.28 ± 0.42

15.03 ± 0.74

2.91 ± 0.21

-

LLA + DLA

3.57 ± 0.22

3.95 ± 0.26

1.11 ± 0.08

-

  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate), DLA or LLA (5 mM + 2.5 mM malate), and LLA+DLA (2.5 mM LLA and 2.5 mM DLA+ 2.5 mM malate).
  3. cCocktail contains 62.5 μeach of malate, glutamate, alpha-ketoglutarate and pyruvate.
  4. State 4: The respiration state without ADP.
  5. State 3: The respiration state with the addition of ADP.
  6. RCR: Respiratory Control Ratio (state 3 rate:state 4 rate).
  7. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  8. * Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test, α < 0.05.
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Nutrition & Metabolism

ISSN: 1743-7075

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