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Table 3 Respiratory parametersa of isolated rat heart mitochondria with pyruvate, D-lactate, L-lactate alone or as combination as substrates.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb State 4 State 3 RCR ADP/O
No substrate 3.07 ± 0.15 7.72 ± 0.55 2.52 ± 0.08  
Cocktailc 11.35 ± 1.52* 70.89 ± 5.89* 6.39 ± 0.59* 2.76 ± 0.02
Pyruvate 12.51 ± 1.19* 74.74 ± 6.22* 5.95 ± 0.22* 3.01 ± 0.02
D-lactate 4.62 ± 0.19 9.27 ± 1.31 2.01 ± 0.27 -
L-lactate 4.58 ± 0.58 15.17 ± 2.67 3.24 ± 0.21 2.11 ± 0.34
Pyruvate + LLA 4.30 ± 0.25 18.23 ± 1.52 4.23 ± 0.15* -
Pyruvate + DLA 5.28 ± 0.42 15.03 ± 0.74 2.91 ± 0.21 -
LLA + DLA 3.57 ± 0.22 3.95 ± 0.26 1.11 ± 0.08 -
  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate), DLA or LLA (5 mM + 2.5 mM malate), and LLA+DLA (2.5 mM LLA and 2.5 mM DLA+ 2.5 mM malate).
  3. cCocktail contains 62.5 μeach of malate, glutamate, alpha-ketoglutarate and pyruvate.
  4. State 4: The respiration state without ADP.
  5. State 3: The respiration state with the addition of ADP.
  6. RCR: Respiratory Control Ratio (state 3 rate:state 4 rate).
  7. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  8. * Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test, α < 0.05.