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Table 5 Respiratory parametersa of isolated rat brain and liver mitochondria with pyruvate, D-lactate, L-lactate as substrates in the presence of CINN, a monocarboxylate transporter inhibitor.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb Tissue State 4 State 3 RCI
Pyruvate + CINN Brain 3.42 ± 0.33 5.88 ± 0.72 1.72 ± 0.13
  Liver 5.16 ± 0.19 10.08 ± 0.74 1.97 ± 0.21
LLA + CINN Brain 2.87 ± 0.04 3.83 ± 0.52 1.34 ± 0.20
  Liver 2.89 ± 0.29 3.68 ± 0.15 1.32 ± 0.20
DLA + CINN Brain 2.46 ± 0.29 2.79 ± 0.23 1.16 ± 0.23
  Liver 2.50 ± 0.40 2.52 ± 0.16 1.03 ± 0.10
  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate) and LLA or DLA (5 mM + 2.5 mM malate); CINN, α-cyano-4-hydroxycinnamate (5 mM).
  3. State 4: The respiration state without ADP.
  4. State 3: The respiration state with the addition of ADP.
  5. RCR, Respiratory Control Ratio (state 3 rate:state 4 rate).
  6. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  7. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test. α < 0.05
  8. Note: Refer to Table 1 and 2 for the respiratory parameters without substrates or with cocktail.