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Table 5 Respiratory parametersa of isolated rat brain and liver mitochondria with pyruvate, D-lactate, L-lactate as substrates in the presence of CINN, a monocarboxylate transporter inhibitor.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb

Tissue

State 4

State 3

RCI

Pyruvate + CINN

Brain

3.42 ± 0.33

5.88 ± 0.72

1.72 ± 0.13

 

Liver

5.16 ± 0.19

10.08 ± 0.74

1.97 ± 0.21

LLA + CINN

Brain

2.87 ± 0.04

3.83 ± 0.52

1.34 ± 0.20

 

Liver

2.89 ± 0.29

3.68 ± 0.15

1.32 ± 0.20

DLA + CINN

Brain

2.46 ± 0.29

2.79 ± 0.23

1.16 ± 0.23

 

Liver

2.50 ± 0.40

2.52 ± 0.16

1.03 ± 0.10

  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate) and LLA or DLA (5 mM + 2.5 mM malate); CINN, α-cyano-4-hydroxycinnamate (5 mM).
  3. State 4: The respiration state without ADP.
  4. State 3: The respiration state with the addition of ADP.
  5. RCR, Respiratory Control Ratio (state 3 rate:state 4 rate).
  6. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  7. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test. α < 0.05
  8. Note: Refer to Table 1 and 2 for the respiratory parameters without substrates or with cocktail.
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Nutrition & Metabolism

ISSN: 1743-7075

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