Skip to main content

Table 3 Relative PC and PDH activities determined from adipocyte exposure to U 13 C-glucose

From: Peroxisome proliferator-activated receptor gamma modulation and lipogenic response in adipocytes of small-for-gestational age offspring

A.

Ā 

Ī£mn C2-4 fragment (meanā€‰Ā±ā€‰SEM)

Ī£mn C2-5 fragment (meanā€‰Ā±ā€‰SEM)

Ā 

Control

Ā 

SGA

Control

Ā 

SGA

Baseline

0.056ā€‰Ā±ā€‰0.003

Ā 

0.073ā€‰Ā±ā€‰0.001C

0.060ā€‰Ā±ā€‰0.003

Ā 

0.084ā€‰Ā±ā€‰0.001C

Rosi Treatment

0.073ā€‰Ā±ā€‰0.002*

Ā 

0.096ā€‰Ā±ā€‰0.002*C

0.083ā€‰Ā±ā€‰0.003*

Ā 

0.110ā€‰Ā±ā€‰0.002*C

B.

Ā 

PC (m2 152) (median with 25-75%)

PDH (m2 198 ā€“ m2 152) (median with 25-75%)

PC/PDH (median with 25-75%)

Control

SGA

Control

SGA

Control

SGA

Control

Baseline

0.015 (0.015-0.017)

0.018 (0.017-0.018)

0.009 (0.009-0.010)

0.017 (0.016-0.018)

1.66 (1.64-1.75)

0.92 (0.90-1.04)C

Rosi Treatment

0.019 (0.018-0.028)

0.025 (0.025-0.026)

0.021 (0.021-0.022

0.021 (0.021-0.022)

1.10 (1.10-1.19)*

1.17 (1.14-1.18)*i

  1. Cpā€‰<ā€‰0.05 compared with Control receiving the same treatment.
  2. *pā€‰<ā€‰0.05 compared to the untreated state within the same group.
  3. i interaction between SGA and rosiglitazone.
  4. A) The Ī£mn representing the average fraction of 13ā€‰C atoms/molecule for the C2-4 fragment and C2-5 fragments are shown for cultures with and without rosiglitazone treatment. The data are presented as fractions of enrichmentā€‰Ā±ā€‰the SEM. Enrichment increased with rosiglitazone treatment in both Controls and SGA, while SGA maintained higher levels than Controls regardless of treatment. B) The PC and PDH pathway contributions are represented based on glutamate fragment enrichments. These data are presented as the median with the 25-75% interquartile ranges. At baseline, SGA demonstrated a lower PC/PDH ratio than Controls. With rosiglitazone, Controls decreased the PC/PDH ratio, while SGA increased it.