Figure 4

Mean ± SEM rat liver gamma-butyrobetaine hydroxylase (Bbh) (A) mRNA expression and (B) activity at postnatal day 4, 8, 11, and 20 (n = 6). mRNA expression was normalized to β-actin and fold difference (FD) was determined by using 2^-ΔΔCT method. Bbh activity was measured by quantifying the conversion of γ-butyrobetaine to L-carnitine by HPLC-UV. One-way ANOVA with Tukey’s post hoc test was used for the comparisons between ages and bars with the same letters indicate no significant difference, P < 0.05.