Glucose tolerance tests on male C57Bl/6 mice fed Zero-CHO and standard Chow diets. Glucose tolerance tests were administered at week 12 of the diet trial. Blood glucose concentrations are shown at 0 min, 30 min, 60 min, and 180 min time points: Chow group (blue), Zero-CHO group (red). Statistical differences among the means were determined using unpaired Student t-Test at each time point (P<0.0009). The inset to the figure shows that every animal in the zero-CHO group showed decreased glucose clearance.