Effects of SFN on aortic expression of Nrf2. Aortic expression of Nrf2 was examined by immunohistochemical staining for the expression of Nrf2 in aortic tunica media with semi-quantitative analysis (Staining for 3 month & 6 month sections were not at same time so that control staining intensity was different between two time-points). Data were presented as means ± SD (n = 6).*, p < 0.05 vs. control; #, p < 0.05 vs. DM. Bar = 50 μM.