Relative mitochondrial DNA content assessment. HF, mice fed high-fat diet; HF-A, mice fed high-fat diet with ALA. (a. epididymal fat; b, inguinal fat). mtDNA, mitochondrial DNA; nDNA, nuclear DNA; % of nDNA, mtDNA content relative to nDNA content. The values are means ± SE. #P < 0.05 for difference between different genotypes with HF-A diet; *P < 0.05 for difference between WT mice.