Effect of hRFVT-2 N- and C- terminal tail truncated constructs on RF uptake and on cellular distribution in U87 cells. A) Schematic representation of the full-length hRFVT-2, and the N- and the C-terminal truncated constructs with GFP fused to the N- terminus (GFP-hRFVT-2). B)
3H-RF uptake (14 nM) assay in a WT and truncated constructs transiently expressing U87 cells. Data represents the means ± SE of at least 3 independent experiments. *P < 0.01, **P < 0.02. C) Western blot was performed using 60 μg of total protein isolated from the wild-type, and truncated constructs expressing U87 cell-lysates. The blot was probed with the anti-GFP monoclonal and β-actin polyclonal primary antibodies. Data (means ± SE) from 3 independent sets of experiment are expressed in arbitrary units. Inset is the image of a representative gel. **P < 0.05. D) Co-expressing GFP-hRFVT-2 (WT) (left) along with DsRed-ER (middle), shown as overlaid image (right). Images were obtained after 24–48 hrs of post-transfection.