Identification of residues/sequences in the human riboflavin transporter-2 that is important for function and cell biology

Table 2 Primer pairs used for generating the full-length, N- and C-terminal tail truncated constructs of hRFVT-2 by PCR

Construct	Forward and reverse primers (5’-3’)	Positions (bp)	Fragment size (bp)
GFP-hRFVT-2[1–445]	CCCAAGCTTATGGCAGCACCCACGCCCGCC; TCCCCGCGGGGAGTCACAGGGGTCTGCACA	1-1335	1335
GFP-hRFVT-2[1–424]	CCCAAGCTTATGGCAGCACCCACGCCCGCC; TCCCCGCGGGAACATAGCAACAGCGCC	1-1272	1272
GFP-hRFVT-2[10–445]	CCCAAGCTTATGGTGCTGACCCACCTGCTG; TCCCCGCGGGGAGTCACAGGGGTCTGCACA	28- 1335	1307
GFP-hRFVT-2[10–424]	CCCAAGCTTATGGTGCTGACCCACCTGCTG; TCCCCGCGGGAACATAGCAACAGCGCC	28-1272	1244

Combination of primers and primer sequence used to generate each construct are shown. The restriction enzyme sites Hind III (boldface italic text) and Sac II (boldface text) were added to the hRFVT-2 primer to enable subsequent sub-cloning into green fluorescent protein (GFP-C3) vector.

ISSN: 1743-7075