Figure 3

Tobacco smoke exposure dysregulates the expression of ADPN chaperones. Panel A shows representative immunoblots for time-dependent effects of TSE on the expression of ERp44, Ero1-Lα, and DsbA-L in 3T3-L1 adipocytes exposed to 1.5% TSE for 0-20 h. Panel B shows confocal fluorescent micrographs of representative 3T3-L1 cells that were stained simultaneously with anti-ERp44 (red) and anti-ADPN (green) antibodies, as well as DAPI (blue; nuclear stain). The yellow color in the merged images (Merge) demonstrates co-localization of ERp44 and ADPN in the ER around the nucleus.