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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: Inflamed macrophage microvesicles induce insulin resistance in human adipocytes

Fig. 1

Detection of macrophage polarization. a. Morphology of THP-1 monocytes, M0 THP-1, M2 THP-1, and M1 THP-1 cells were examined by light microscopy (100×). b, c. Phenotypic gene expression in M0 THP-1, M1 THP-1, and M2 THP-1 cells was analyzed by qRT-PCR. d. Expression of CD11c and CD206 was determined by flow cytometry. Quantification of the flow cytometry assay was analyzed using FlowJo software, and statistical analysis is presented below. e, f. IL-10 (e) and IL-1β level (f) in the cell culture supernatant were analyzed by ELISA. **P < 0.01 indicates a significant difference compared with the corresponding vehicle groups. ***P < 0.001 indicates a significant increase when compared with the indicated conditions

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