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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Inflamed macrophage microvesicles induce insulin resistance in human adipocytes

Fig. 3

Effects of macrophage-derived MVs on insulin-stimulated Akt phosphorylation in human mature adipocytes. a. The level of pAkt in human mature adipocytes after treatment with various concentrations of M0 MVs, M1 MVs, and M2 MVs was detected by western blot assays. Ethanol treatment was used as the negative control, and the palm group was the positive control. Western blot images were analyzed using Bandscan software, and statistical analysis is presented below. b. Levels of insulin-stimulated pAkt protein in adipocytes after treatment with indicated MVs were determined by western blotting. PBS treatment was used as the basal control. Quantification of the pAkt protein levels is shown below. **P < 0.01 indicates a significant enhancement compared with the corresponding vehicle control. #P < 0.05 indicates a significant difference when compared with the indicated conditions

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