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Fig. 7 | Nutrition & Metabolism

Fig. 7

From: Inflamed macrophage microvesicles induce insulin resistance in human adipocytes

Fig. 7

Blocking of NF-κB reverse the inhibitory effect of M1 MVs on pAkt level and GLUT4 translocation in human adipocytes. a, d. Expression level of nuclear NF-κB p65 in human mature adipocytes (a) and differentiated cells (d) were analyzed by western blotting. Adipocytes were pretreated with or without NF-κB specific inhibitor (Bay, 10 μM) for 2 h, then incubated with M1 MVs for 24 h. Nuclear lysates were analyzed for NF-κB p65 and histone H3 level after insulin stimulation for a further 20 min. Quantification of NF-κB p65 protein level is presented below. b, e. Protein level of pAkt in human mature adipocytes (b) and differentiated adipocytes (e), after treatment as in a. Western blot images were analyzed using Bandscan software, and statistical analysis is presented below. c, f. The levels of GLUT4 in plasma membrane (PM) fractions of human mature adipocytes (c) and differentiated adipocytes (f) were determined by western blotting. Cells were treated as in A, and PM fractions were obtained and subjected to immunoblotting with the indicated antibodies. Quantification of the GLUT4 protein level is shown below. *P < 0.05 indicates a significant difference compared with corresponding vehicle-treated control

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