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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: A novel anti-inflammatory natural product from Sphaeranthus indicus inhibits expression of VCAM1 and ICAM1, and slows atherosclerosis progression independent of lipid changes

Fig. 1

Sphaeranthus Indicus extract inhibits lipopolysaccharide-induced TNF-α production in human peripheral monocytic cells. Freshly isolated human peripheral blood monocytes were pretreated with S. indicus extract ranging in concentration from 0.01 to 100 μg/ml for 30 min followed by treatment with LPS and incubation for 6 h at 37 °C, 5 % CO2 followed with collection of supernatants and measurements for TNF-α, IL-6, IL-8, and IL-1β by ELISA Assay. The 50 % inhibitory concentration (IC50) values were calculated by a nonlinear regression method using GraphPad software

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