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Fig. 5 | Nutrition & Metabolism

Fig. 5

From: Ammonia-lowering activities and carbamoyl phosphate synthetase 1 (Cps1) induction mechanism of a natural flavonoid

Fig. 5

NOB coordinately enhanced urea cycle gene expression. a Diagram of C/EBP sites on the genomic regions for urea cycle genes including Otc, Ass1, Asl, and Arg1. b-e Liver samples were collected from the four diet/treatment groups of wild-type mice at the indicated circadian time points. Total RNAs were purified for real-time RT-PCR analysis using primers for the four urea cycle genes, including Otc (b), Ass1 c, Asl d and Arg1 e. RC regular chow, HFD high-fat diet, Veh vehicle, NOB Nobiletin. Data are presented as mean ± SEM (n = 3). Two-way ANOVA with Bonferroni post-hoc tests, RC.Veh vs. HFD.Veh, a p < 0.0001, b p < 0.01, c p < 0.05, d p < 0.01. Student’s t-test, HFD.Veh vs. HFD.NOB, *p < 0.05, **p < 0.01

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