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Fig. 6 | Nutrition & Metabolism

Fig. 6

From: High-fructose and high-fat diet-induced insulin resistance enhances atherosclerosis in Watanabe heritable hyperlipidemic rabbits

Fig. 6

Quantitation of aortic atherosclerosis at 8 weeks after HFFD feeding. a Representative pictures of aortas stained with Sudan IV are shown on the left. The lesion area (defined by sudanophilic staining as red) was quantified using an image analysis system (right). Data are expressed as the mean ± SD. n = 9-10 for each group. *P < 0.05 vs. the control group. b Representative micrographs of the aortic arch lesions from each group. Serial paraffin sections were stained with hematoxylin-eosin (HE) and elastica van Gieson (EVG) or immunohistochemically stained with mAbs against either macrophages (Mϕ) or α-smooth muscle actin for smooth muscle cells (SMCs). The lesions are characterized by intimal accumulation of macrophage-derived foam cells intermingled with smooth muscle cells. c Quantitation of the lesions of different parts of aortas. The intimal lesion area and positively immunostained area of macrophages and SMCs were quantified using an image analysis system as described in the Methods. Data are expressed as the mean ± SD. n = 9-10 for each group. *P < 0.05 vs. the control group

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