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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Quercetin reduces obesity-induced hepatosteatosis by enhancing mitochondrial oxidative metabolism via heme oxygenase-1

Fig. 3

Effects of quercetin on lipid accumulation and HO-1 induction in hepatocytes. a-b Mouse primary hepatocytes were incubated with a 1 mM FFA mixture (2:1 ratio of oleate/palmitate) for 24 h, and exposed to various concentrations of quercetin. N-hepa was added with 100 μM BSA. N-hepa; normal hepatocyte, F-hepa; fatty hepatocytes. a Intracellular TG content. Data are mean ± SEM of quadruplicate samples. *p < 0.05, ## p < 0.001 versus F-hepa. b HO-1 protein was determined by Western blotting. Data are mean ± SEM from two independent experiments. *p < 0.05, **p<0.01 versus F-hepa. c-d Mouse primary hepatocytes from Nrf-2 deficient and WT mice were incubated with 1 mM FFA mixture (2:1 ratio of oleate/palmitate) for 24 h and exposed 10 μM quercetin and/or 0.1 μM ZnPP, an HO-1 inhibitor. Control cells were incubated with FFA mixture. c Wild type lipid-laden hepatocytes. d Nrf-2 deficient lipid-laden hepatocytes. Expressions of mRNAs associated with lipid oxidation (PPARα and CPT-1α) and mitochondrial biogenesis (PGC-1α) were quantified by real-time PCR. Data are mean ± SEM from three independent experiments. *p < 0.05, ## p < 0.001 versus Que. e HO-1 protein was determined by Western blotting. Data are mean ± SEM from four independent experiments. *p < 0.05 versus WT-Con

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