Fig. 1
Hepatic S1P KD and plasma lipid levels. Hepatic S1P KD was verified by (a), Reverse transcriptional PCR analysis of liver cDNA using primers that binds to sequence outside of the floxed sites; (b), Real-time PCR analysis of S1P KD in hepatocytes from S1Pf/fCre and WT control mice. N = 4, each data point represents the mean +/- S.D., * denotes p < 0.05; (c), VLDL-TG secretion study. Four-hour fasted female S1Pf/f Luci and S1Pf/fCre mice were injected intraperitoneally with poloxamer 407 (P407) and plasma TGs monitored over a period of 6 h. N = 4, 6–8 weeks of age each data point represents the mean +/- S.D., * denotes p < 0.05; (d-e), Lipid measurements were carried out on gel filtration fractions from the pooled plasma samples. This experiment was repeated three times and similar results were obtained