Fig. 5
S1P regulates the rate of PCSK9 transcription. a, Comparison of PCSK9 mRNA levels at the indicated time points in S1Pf/f Luci and S1Pf/fCre hepatocytes after treatment with actinomycin D (10 μg/ml). n = 6, values plotted as % of the 0 h value; b, Comparison of nuclear synthesis of nascent PCSK9 transcripts in S1Pf/f Luci and S1Pf/fCre hepatocytes using a run-on assay. Values are plotted as the mean of triplicate measurements from one representative experiment. The experiment was repeated three times yielding similar results; c and d, PCSK9 promoter activities in S1Pf/fLuci and S1Pf/fCre hepatocytes. N = 3, each data point represents the mean +/- SD, * denotes p < 0.001