Fig. 3

RNA expression of MCP-1 and TGF-β was upregulated in the kidneys of offspring of obese mothers at Day 1 but not at Day 20. Collagen IV but not fibronectin mRNA was increased in offspring of obese mothers at Day 1 but not at Day 20. However, cortical protein expression of fibronectin and Collagen IV was increased in offspring of obese mothers at Day 20. Renal mRNA expression of MCP-1 (a), TGF-β1 (b), Collagen IV (c) and fibronectin (d) at postnatal Day 1 and Day 20. Protein expression of fibronectin (e) and Collagen IV (g) in offspring of obese mothers at Day 20. Graphical quantitation of fibronectin and Collagen IV staining are shown in (f) and (h) respectively. N = 5–7 per group, *p <0.05, *p <0.01