Fig. 5

Effect of ezetimibe and simvastatin on genes and protein expression. The expression levels of (a) srebf2, hmgcs1 and hmgcra (b) pparab, cpt1a and acox3 (c) atf6, hspa5 and hsp90b1 in 2.5 % HFC group, 2.5 % HFC + ezetimibe group and 2.5 % HFC + simvastatin group were compared with the gene expression in control group. Gene expression analysis using cDNA prepared from pools of livers dissected from larvae (n = 20-30) in each group after 10 days of feeding. Fold change in GRP78/BiP protein levels normalizing to β-actin was examined by western blot in the livers of zebrafish larvae. Data are expressed as mean ± SD, N.S.: no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA