Fig. 1

Experimental design. Female mice were either not subjected (a) or subjected (b) to an exercise adaptation period for 14 days followed by mating time (1-5 days). Group (b) was then exercising before and during pregnancy (1 h/day, 5 days/week) for 18-21 days (depending on the length of pregnancy). Male and female offspring of both groups was fed standard diet (SD) until 12 weeks of age. Afterwards, pups received HFD over a period of 16 weeks. At 28 weeks, animals were sacrificed and the tissue was used for a variety of analysis. (Notably, only male offspring was tested for indirect calorimetry, hormone levels and gene expression)