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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Effects of low-stearate palm oil and high-stearate lard high-fat diets on rat liver lipid metabolism and glucose tolerance

Fig. 2

Determination of total IHCL content and 13C enrichment of IHCL by MRS. Typical examples of unedited LASER-POCE spectra acquired from the liver of animals from each diet group after 10 weeks of diet feeding (a), and LASER-POCE spectra with and without 13C editing acquired from the liver of a HFL rat at 24 h after 13C-labeled lipid administration, together with the calculated difference spectrum containing only 13C-coupled 1H resonances (5x magnification) (b). Total lipid content at baseline (c) and absolute 13C-labeled IHCL content corrected for 13C natural abundance at 4 and 24 h after the oral administration of [U-13C] algal lipid mixture (d) in rats on normal chow control diet (CON), high-fat palm oil diet (HFP), and high-fat lard diet (HFL) (n = 9 per group). H2O, residual water; Cho, choline; IHCL, intrahepatocellular lipids. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CON; † p < 0.05 vs. HFP; § p < 0.05, §§ p < 0.01, §§ p < 0.001 vs. 4 h

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