Fig. 4

A-Mttp ^−/− mice have smaller size adipocytes and less macrophages infiltration in the adipose tissue. Control Mttp ^fl/fl and A-Mttp ^−/− mice were fed 60 % high fat diet for 15 and 24 weeks. At the end of experiment, adipose tissue was collected for H & E staining (a), adipocyte size determination (b), and cell number quantification (c). Adipose tissue was isolated after collagenase treatment and counted under microscope. Immunohistochemical detection of cells expressing the macrophage-specific antigen F4/80 (arrows) was performed on paraffin section of adipose tissue in control Mttp ^fl/fl and A-Mttp ^−/− mice (d)