Fig. 2

Combined treatment with DHA and AC increased insulin-stimulated adipocyte lipogenesis. Basal and insulin-stimulated lipogenesis measured in cell lysates after 48 h of treatment with bioactive compounds. Control, n = 8; 0.5 μM DHA, n = 6; AC alone and with DHA, n = 3; 100 μM PI alone, n = 3 and with DHA, n = 4 biological/independent experiments in duplicates. Glucose incorporation into lipids (3-³H counts per minute) is normalized for protein concentration in the cell lysates for each well. Normalized data is presented as means +/- standard deviation. *p < 0.05, **p < 0.01 and ***p < 0.001 counted against basal control with the same bioactive treatment or insulin-stimulated control compared with insulin-stimulated bioactive treated cells. Statistical significance was determined by the non-parametric Kruskal-Wallis test with subsequent Mann-Whitney multiple comparisons post hoc test