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Table 3 Gene expression analyses by qRT-PCR for diet comparison in Acads−/− mice

From: Short chain acyl-CoA dehydrogenase deficiency and short-term high-fat diet perturb mitochondrial energy metabolism and transcriptional control of lipid-handling in liver

   Microarray qRT-PCR
Gene Symbol Gene Name Fold change p-value Fold change p-value
Aacs acetoacetyl-CoA synthetase −3.34 0.000 −3.34 0.001
Abcc3 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 1.98 0.004 2.07 0.003
Acaa1a acetyl-Coenzyme A acyltransferase 1A 1.82 0.003 1.59 0.041
Acot1 acyl-CoA thioesterase 1 3.11 0.039 4.15 0.011
Apol9b apolipoprotein L 9b 3.00 0.022 2.34 0.000
Decr2 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal 2.04 0.003 2.04 0.011
Dhrs13 dehydrogenase/reductase (SDR family) member 13 1.82 0.046 1.42 0.018
Ehhadh enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase 1.64 0.025 1.93 0.014
Elovl6 ELOVL family member 6, elongation of long chain fatty acids (yeast) −24.79 0.021 −11.11 0.000
Fasn fatty acid synthase −7.21 0.000 −6.69 0.000
Gpam glycerol-3-phosphate acyltransferase, mitochondrial −5.70 0.000 −2.26 0.000
Gpi1 glucose phosphate isomerase 1 −2.49 0.001 −2.16 0.000
Gpr146 G protein-coupled receptor 146 2.03 0.003 2.17 0.000
Gstm2 glutathione S-transferase, mu 2 −2.33 0.002 −1.76 0.000
Macrod2 MACRO domain containing 2 −1.97 0.005 −1.11 0.444
Mod1/Me1 malic enzyme 1, NADP(+)-dependent, cytosolic −5.81 0.025 −5.35 0.000
Nars asparaginyl-tRNA synthetase −1.65 0.025 −1.49 0.133
Pdhb pyruvate dehydrogenase (lipoamide) beta −1.83 0.015 −1.58 0.007
Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1 −1.72 0.004 −1.54 0.307
Pdk4 pyruvate dehydrogenase kinase, isoenzyme 4 −3.98 0.018 −2.00 0.004
Pklr pyruvate kinase liver and red blood cell −5.28 0.000 −5.28 0.000
Rps6kl1 ribosomal protein S6 kinase-like 1 2.61 0.015 1.63 0.026
Scd1 stearoyl-Coenzyme A desaturase 1 −8.57 0.024 −19.17 0.000
Slc17a4 solute carrier family 17 (sodium phosphate), member 4 −2.74 0.009 −1.91 0.000
Slc27a5 solute carrier family 27 (fatty acid transporter), member 5 1.62 0.004 1.77 0.000
Sucnr1 succinate receptor 1 −2.33 0.033 −2.55 0.379
Uox urate oxidase −1.65 0.015 −1.33 0.039
  1. Representative genes were selected to validate the microarray results in liver, using real-time RT-PCR. For the comparison of Acads−/− HF diet vs. LF diet, 27 genes are shown. There was strong concordance between the two platforms, i.e., 23 PCR assays reached statistical significance in a two-tailed Student’s t-test performed on ΔCt values