Short chain acyl-CoA dehydrogenase deficiency and short-term high-fat diet perturb mitochondrial energy metabolism and transcriptional control of lipid-handling in liver

Ghosh, Sujoy; Kruger, Claudia; Wicks, Shawna; Simon, Jacob; Kumar, K. Ganesh; Johnson, William D.; Mynatt, Randall L.; Noland, Robert C.; Richards, Brenda K.

doi:10.1186/s12986-016-0075-0

Table 3 Gene expression analyses by qRT-PCR for diet comparison in Acads−/− mice

From: Short chain acyl-CoA dehydrogenase deficiency and short-term high-fat diet perturb mitochondrial energy metabolism and transcriptional control of lipid-handling in liver

		Microarray		qRT-PCR
Gene Symbol	Gene Name	Fold change	p-value	Fold change	p-value
Aacs	acetoacetyl-CoA synthetase	−3.34	0.000	−3.34	0.001
Abcc3	ATP-binding cassette, sub-family C (CFTR/MRP), member 3	1.98	0.004	2.07	0.003
Acaa1a	acetyl-Coenzyme A acyltransferase 1A	1.82	0.003	1.59	0.041
Acot1	acyl-CoA thioesterase 1	3.11	0.039	4.15	0.011
Apol9b	apolipoprotein L 9b	3.00	0.022	2.34	0.000
Decr2	2-4-dienoyl-Coenzyme A reductase 2, peroxisomal	2.04	0.003	2.04	0.011
Dhrs13	dehydrogenase/reductase (SDR family) member 13	1.82	0.046	1.42	0.018
Ehhadh	enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase	1.64	0.025	1.93	0.014
Elovl6	ELOVL family member 6, elongation of long chain fatty acids (yeast)	−24.79	0.021	−11.11	0.000
Fasn	fatty acid synthase	−7.21	0.000	−6.69	0.000
Gpam	glycerol-3-phosphate acyltransferase, mitochondrial	−5.70	0.000	−2.26	0.000
Gpi1	glucose phosphate isomerase 1	−2.49	0.001	−2.16	0.000
Gpr146	G protein-coupled receptor 146	2.03	0.003	2.17	0.000
Gstm2	glutathione S-transferase, mu 2	−2.33	0.002	−1.76	0.000
Macrod2	MACRO domain containing 2	−1.97	0.005	−1.11	0.444
Mod1/Me1	malic enzyme 1, NADP(+)-dependent, cytosolic	−5.81	0.025	−5.35	0.000
Nars	asparaginyl-tRNA synthetase	−1.65	0.025	−1.49	0.133
Pdhb	pyruvate dehydrogenase (lipoamide) beta	−1.83	0.015	−1.58	0.007
Pdk1	pyruvate dehydrogenase kinase, isoenzyme 1	−1.72	0.004	−1.54	0.307
Pdk4	pyruvate dehydrogenase kinase, isoenzyme 4	−3.98	0.018	−2.00	0.004
Pklr	pyruvate kinase liver and red blood cell	−5.28	0.000	−5.28	0.000
Rps6kl1	ribosomal protein S6 kinase-like 1	2.61	0.015	1.63	0.026
Scd1	stearoyl-Coenzyme A desaturase 1	−8.57	0.024	−19.17	0.000
Slc17a4	solute carrier family 17 (sodium phosphate), member 4	−2.74	0.009	−1.91	0.000
Slc27a5	solute carrier family 27 (fatty acid transporter), member 5	1.62	0.004	1.77	0.000
Sucnr1	succinate receptor 1	−2.33	0.033	−2.55	0.379
Uox	urate oxidase	−1.65	0.015	−1.33	0.039

Representative genes were selected to validate the microarray results in liver, using real-time RT-PCR. For the comparison of Acads−/− HF diet vs. LF diet, 27 genes are shown. There was strong concordance between the two platforms, i.e., 23 PCR assays reached statistical significance in a two-tailed Student’s t-test performed on ΔCt values

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ISSN: 1743-7075

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