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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: The role of lipid droplet formation in the protection of unsaturated fatty acids against palmitic acid induced lipotoxicity to rat insulin-producing cells

Fig. 2

Lipid droplet formation in rat insulin-producing RINm5F cells after incubation with unsaturated NEFAs with a different degree of unsaturation. RINm5F cells were incubated with C18:x (a), C20:x (b) and C22:x (c) NEFAs (100 μM) with different numbers of double bonds for 24 h. Thereafter cells were fixed and stained with Oil Red O. Lipid droplet formation was analyzed by fluorescence microscopy and quantified using the software xcellence rt. Data are given as means ± SEM from 5 to 8 individual experiments. **p < 0.01, ***p < 0.001 compared with control cells (Dunnett’s Multiple Comparison Test)

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