Fig. 3

Lipid droplet formation in co-incubation experiments with unsaturated NEFAs in combination with increasing PA concentrations. RINm5F cells were incubated with 100 μM of the unsaturated NEFAs C18:1 (a) or C20:1 (b) in combination with increasing concentrations of the saturated NEFA C16:0 for 24 h. Thereafter cells were fixed, stained with Oil Red O and analyzed by fluorescence microscopy. Lipid droplet formation per cell was quantified by the software xcellence rt. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control cells (Dunnett’s Multiple Comparison Test). ^# p < 0.05, ^## p < 0.01, ^### p < 0.005 compared to unsaturated NEFA only (Student’s t-test)