Fig. 4

Gene expression analysis of perilipin 1 or 2 suppressed RINm5F and INS-1E cells. Perilipin 1 and 2 expression in RINm5F (a, c) and INS-1E (b, d) cells was stably suppressed by the shRNA technique after lentiviral transduction. Cells were incubated with 100 μM palmitic acid (PA), 100 μM oleic acid (OA), a combination of both, or 100 μM gondoic acid (GA) or without NEFAs (Con) for 24 h. Thereafter RNA was isolated and reverse transcribed into cDNA. Gene expression of perilipin 1 and perilipin 2 was measured by qRT-PCR and normalized to the reference genes PPIA, α-tubulin and β-actin using qBase + software (Biogazelle). Data are means ± SEM of n = 4. ^* p < 0.01 compared to control cells without NEFAs (ANOVA/Tukey Multiple Comparison Test). ^# p < 0.01 compared with control cells (ANOVA/Tukey Multiple Comparison Test)