Fig. 6

Effect of perilipin 1 or 2 suppression in insulin-producing RINm5F and INS-1E cells on lipid droplet formation after OA and PA incubation. For the suppression of perilipin 1 and 2 RINm5F (a, c) and INS-1E (b, d) cells were transduced lentivirally with the respective shRNA constructs or as a control with a non-target shRNA (shNTC). Cells were incubated with the indicated NEFAs for 24 h and after fixation stained with Oil Red O. Lipid droplet formation was analyzed by fluorescence microscopy and quantified using the software xcellence rt. a, b Cells were incubated with 100 μM oleic acid (C18:1, OA) or under control conditions (Con). Data are presented as means ± SEM of 4 to 5 independent experiments. ^## p < 0.01; ^### p < 0.001 compared with shNTC cells after incubation with OA (ANOVA/Tukey Multiple Comparison Test). ***p < 0.001 compared with non-OA-incubated controls (ANOVA/Tukey Multiple Comparison Test). c, d Cells were incubated with 200 μM palmitic acid (PA) in combination with 100 or 200 μM OA. Data are presented as means ± SEM of 4 independent experiments. *p < 0.05; compared with shNTC cells with the respective PA and OA concentrations (ANOVA/Tukey Multiple Comparison Test)