Fig. 3

Transcripts of thermoregulatory genes in inguinal adipose tissue. The transcripts for UCP1 a, CIDEA b, and Cox8b (c) were evaluated in inguinal adipose tissue from mice maintained at room temperature (22 °C) or exposed to cold (5 °C) for one week in 3 separate experiments where the ages of the mice at the end of the experiments were 2 months and 10 days (Exp-1), 2 months and 23 days (Exp-2), and 4 months and 10 days (Exp-3). Transcripts were normalized to 22 °C in Experiment-1. Values are means ± SEM (n = 4–5). Comparisons of 22 °C vs 5 °C within each experiment for all transcripts were statistically significantly different (P ≤ 0.05) and are not marked with a symbol. (*) different from Exp-3, 22 °C, (#) different from Exp-2, 5 °C, (+) different from Exp-3, 5 °C, (@) different from Exp-1, 5 °C, p ≤ 0.05, two way ANOVA followed by multiple comparisons using the Holm-Sidak method