Fig. 5

Effects of APE on GLUT4 protein levels in L6 myotubes. a Cells were deprived of FBS for 18 h and incubated in the absence or presence of 25 μg/mL APE for 18 h. GLUT4 protein amount was assayed by Western blot in cell lysates and plasma membrane fractions. Signal densities from control cells were assigned a value of 100 % for the transporter. Effects of APE on 2-DG uptake. b L6 myotubes were deprived of FBS for 18 h and then incubated with increasing amounts of APE (0–50 μg/mL) for 2 h. Results are mean ± SEM of five independent experiments. (a) p < 0.05 compared with control non-treated cells